Novici’s patented GRAMMR™ technology is a novel molecular evolution platform that can generate large libraries of extensively shuffled gene sequences faster and more efficiently than traditional approaches. Novici can deliver both gene and protein libraries to clients for incorporation into product development programs.
 
Novici achieves library superiority by focusing on the regions of DNA sequence diversity rather than areas of sequence identity, which allows us to evolve even highly divergent molecules. In GRAMMR™ shuffling, genes remain full-length and the chimeric genes are generated by enzymatic resolution of mismatches in heteroduplexes formed from different parental gene DNAs. For more information on GRAMMR, click here.
 
The competitive advantages of GRAMMR™ include:
  • A greater variety of chimeric products from fewer rounds of shuffling
  • Very high crossover frequency and uniform crossover site distribution
  • Rapid generation of many thousands of shuffled genes in a single day
  • Works on very large genes as well as multiple gene constructs
  • Capable of shuffling even highly divergent genes
  • No gene recloning required
  • Low background of ‘junk’ sequences means improved screening efficiencies
  • Supported by custom informatics package to seamlessly manage samples and data
 
Benefits
  • Explands your access to combinatorial space at lower cost
  • Produces shuffled libraries quickly and reliably
  • Robust and reliable production of shuffled libraries allow resources to be focused on screening instead of producing molecular libraries
 
Intellectual Property - Novici holds a number of US and foreign patents covering GRAMMR technology
 
 
 
A Synthetic Gene Error Correction Technology
  • Dramatically reduces (greater than 10–fold) errors in gene synthesis
  • Allows synthesis of larger genes with fewer errors
  • Saves time and reduces the overall cost of gene synthesis
  • Available from Novici in kit form or by custom bulk order

For more information and orders, click here.

 
 
High-throughput eukaryotic protein expression and screening system allows us to…
  • Express proteins in a higher eukaryotic system
  • Produce proteins that are secreted or targeted to subcellular locations
  • Express and screen tens of thousands of variant clones in a variety of convenient formats
  • Recover and clone corresponding genes after desired proteins are identified
 
 
Novici supports its clients needs with additional capabilities that support the practical use of its proprietary technology. These include…
 
Gene expression
  • Bacterial expression
  • Eukaryotic expression
 
Protein purification
  • Extraction and purification from microgram to gram quantities
  • Affinity purification or biochemical separations
 
Assay development and high throughput analysis
  • Plate-based assays
  • Selective screens