Technologies
GRAMMR™
Novici’s patented GRAMMR™ technology is a novel molecular evolution platform that can generate large libraries of extensively shuffled gene sequences faster and more efficiently than traditional approaches. This technical superiority is achieved in part by focusing on the regions of DNA sequence diversity rather than areas of sequence identity, which allows us to evolve even highly divergent molecules. During the GRAMMR™ reassortment process, genes remain full-length, and resulting chimeras are generated by enzymatic resolution of mismatches in heteroduplexes formed from a population of two or more parental molecules.
The competitive advantages of GRAMMR™ include:
- A greater variety of chimeric products from fewer rounds of shuffling
- Very high crossover frequency and uniform crossover site distribution
- Rapid generation of thousands of shuffled gene sequences in a single day
- Suitable for large genes and multiple gene constructs
- Capable of shuffling highly homologous and highly divergent genes
- Improved screening efficiencies
- Integrated technology and informatics platforms result in improved cost effectiveness
Beyond GRAMMR™
Novici’s strong focus on molecular evolution is complemented by our additional expertise in plant viral vector technology and plant-made pharmaceutical (PMP) bioprocessing.
Novici’s innovative research in virus-based manufacturing systems has resulted in significant improvements that can overcome major PMP concerns.
We have successfully engineered biological control mechanisms for:
- Enhanced biocontainment of viral vectors used in biopharmaceutical manufacturing applications.
- Elimination of virus and viral coat protein impurities from plant-made pharmaceutical bioprocess and protein purification workflows.